| Problem |
Possibie reason |
Proposed action |
| Pipette leaks, Uneven liquid level in tips on multi channel pipette |
Pipette tip base and tip are not compatibie |
Change to suitable tip
(e.g. ExpeWM or ExpellPlus™) |
|
Insufficient tip quality |
Change to suitable tip
(e.g. ExpeWM or ExpellPlus™) |
|
Tip rack bends when
mounting tips on a multi
channel pipette |
Change to more solid tip rack |
|
Tip is not firmly attached |
Press on tip more firmly (avoid hammering pipette into tip |
| Inaccuracy, imprecision |
Pipette is not properly calibrated |
Recalibrate |
|
Sealing ring on the inside of the tip collides with the 0-rings on the tip base |
Change to tip without internal sealing ring |
|
Top screw on volume controller (marked 51 in the calibration guide) is loose |
Tighten the screw, recalibrate |
|
O-ringes) on tip base are worn or damaged |
Change o-ring (s) |
|
Piston seal (inside O-ring) is damaged |
Dismount tip base (please follow the instructions listed for Lubrication) and replace O-ring |
|
Tip base is loose |
Tighten the tip base |
| Liquid remaining in tip after blow-out |
Tip used is hydrophilic |
Change to a hydrophobic tip
(e.g. ExpeWM or ExpellPlus™) |
| Uneven movement of piston |
Piston seal (inside o-ring) is damaged |
Dismount tip base (please follow the instructions listed for Lubrication) and replace o-ring |
|
Oil on piston is unevenly
distributed |
Set the pipette at maximum volume and operate the plunger fully up and down 3-5 times |
|
Piston needs lubrication |
Lubricate piston |
|
Deposit on piston |
Clean the piston and re-Iubricate |
